Comparative study of continuous ethanol fermentation from molasses by using Saccharomyces cervisiae and Schizosaccharomyces pombe

Ethanol production has been carried out from molasses by yeast Saccharomyces cervisiae and Schizosaccharomyces pombe. The level of alcohol production from molasses by the use of both these organisms in continuous ethanol fermentation. The highest percentage of alcohol production by Saccharomyces cervisiae with specific gravity 1.032 was 7.20% whereas Schizosaccharomyces pombe showed 5.25% of alcohol production with specific gravity 1.032. Batch fermentation was conducted by these organism for the role of bio protease enzyme in ethanol production. Higher alcohol production was notiied in flask which contained enzymes with Saccharomyces cervisiae and Schizosaccharomyces pombe. It was found that Saccharomyces cervisiae with enzyme added batch fermentation showed 8% of alcohol production is higher compared with free cells as only 6% of alcohol production. Amount of total reducing sugar and fermentation efficiency of the substrate molasses were calculated and tabulated. Molasses is a complex substrate that has wide range of nutrients that are not often completely metabolized by the microbial inoculums. Analysis of COD reduction rate of Saccharomyces cervisiae used waste water was 41.50%. Analysis of COD reduction rate of Schizosaccharomyces pombe used waste water was 50.78%. From this study, we conclude that Saccharomyces cervisiae yielded better results in alcohol production compared to Schizosaccharomyces pombe.